Insertion junction: CLIP2.025507_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.025507
Chromosome:chromosome 17
Location:3922811
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g727900 CRC1 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase; Centrin-related calmodulin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCATCGAGGGCGCAGGGCTGCGCTGGGCCGCCCCAGCAGCCGCCCAA
Internal bar code:ACGCACACAGACGATAAAACTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1681
LEAP-Seq percent confirming:86.6667
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAACATCTGTGTTTGGCG
Suggested primer 2:CACCTCGTATCCAGGGAAGC