Insertion junction: CLIP2.025507_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.025507
Chromosome:chromosome 17
Location:3922822
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g727900 CRC1 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase; Centrin-related calmodulin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGATGCAAGGCGGCGGCGGAGCAGCTAGCGCCGTCTACTGTCCCTGGC
Internal bar code:ACGCACACAGACGATAAAACTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2304
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTCGTATCCAGGGAAGC
Suggested primer 2:TGCAACATCTGTGTTTGGCG