| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.025515 |
| Chromosome: | chromosome 16 |
| Location: | 7053361 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674515 | IDA7,DIC3,IA1-IC140,IC140 | (1 of 1) PTHR12442//PTHR12442:SF5 - DYNEIN INTERMEDIATE CHAIN // WD REPEAT-CONTAINING PROTEIN 63; Flagellar Inner Arm Dynein I1/f Intermediate Chain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGACGACAGCCCCAGCCCCAGCCCAAGCCCAAGCCCGAGCCTAGCATC |
| Internal bar code: | TTCTATTCCTTTGAAGGACGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2327 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACGTTGTAGGGTTGGAGG |
| Suggested primer 2: | CGGAAGTTCCACAGCAGGAT |