Insertion junction: CLIP2.025525_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.025525
Chromosome:chromosome 8
Location:210425
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCTGGCTCCGGCTATGCCACACCCCGCTCCACACACCCGCACGACGTC
Internal bar code:CCTCGTCAGGGCGAAGCGGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1110
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCAGATAACACCGCAATG
Suggested primer 2:CGTAGAACTACCGTCCGCTC