| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.025533 |
| Chromosome: | chromosome 4 |
| Location: | 1749042 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212050 | (1 of 2) IPR000104//IPR000253//IPR008984 - Antifreeze protein, type I // Forkhead-associated (FHA) domain // SMAD/FHA domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGTGGTGGACGTGAGCGTACGCCAGCGTGTGCGCCGTGTGGGCCGTG |
| Internal bar code: | CCAGTATCGATTACTGAATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3218 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTTGGCGGGATAGTTGG |
| Suggested primer 2: | AGAAACGAGGGTCATCGCAG |