| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025552 |
| Chromosome: | chromosome 13 |
| Location: | 2056376 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576800 | PHX21,HIF1,HIF | (1 of 1) K09592 - hypoxia-inducible factor prolyl hydroxylase (EGLN, HPH); Cytosolic prolyl 4-hydroxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGAATTGGGGAGGACACGGGGCAGGCACCCCTGCCTCGGGAGTAGGGA |
| Internal bar code: | TCTTTAGTGTACGACATTGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACCATCCCCGCATTTCCC |
| Suggested primer 2: | TGATGCAGGCACATGGTCAT |