| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025570 |
| Chromosome: | chromosome 12 |
| Location: | 243448 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485150 | GAP1a,GAP1,GAPC1 | (1 of 2) 1.2.1.12 - Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) / Triosephosphate dehydrogenase; Glyceraldehyde 3-phosphate dehydrogenase, GAPC, chloroplastic | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGCAGCATCGTGGCGCGCATAACCAGGCGGCCAATGCGGCCGAAGC |
| Internal bar code: | AGGGGCCGAGGCGATTGCGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3866 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCCAAACTACTCCTGTGT |
| Suggested primer 2: | GAGTAGCGTCGTCGTGCATA |