| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025574 |
| Chromosome: | chromosome 10 |
| Location: | 2778208 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438400 | LTH2 | LAT2 homolog | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCCGTCAACCACGGCTACCTCCCGCCGTCTAACTACGATGCCGGCCG |
| Internal bar code: | TGATTTGTGTTCTCCGTTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2749 |
| LEAP-Seq percent confirming: | 98.4848 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGGCATTTGTGGTTGGC |
| Suggested primer 2: | GGTACAACTGCGGCACAAAC |