Insertion junction: CLIP2.025574_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.025574
Chromosome:chromosome 10
Location:2778210
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g438400 LTH2 LAT2 homolog intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGGTGTTGACCGGTCACGCGCTCATGCACACGCCTCACCGTCGACCCC
Internal bar code:CTATTAATTTTGTTTCTTCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1852
LEAP-Seq percent confirming:95.1219
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:GGTACAACTGCGGCACAAAC
Suggested primer 2:CTCTGGCATTTGTGGTTGGC