Insertion junction: CLIP2.025577_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.025577
Chromosome:chromosome 7
Location:2377626
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g328550 VPS18 Subunit of the VPS-C complex; (1 of 1) PF05131 - Pep3/Vps18/deep orange family (Pep3_Vps18) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGCGGGCGGGACTTTGGGGGCGCGTGGGGCGTGGGGCGGGGTGGACA
Internal bar code:AACAACGAGTGTTGTACAGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:122
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGGATCTGACAGGAGGGAGG
Suggested primer 2:TACGAAGGACAAACCCAGGC