| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.025584 |
| Chromosome: | chromosome 12 |
| Location: | 3012659 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501850 | FFT1 | putative fructan fructosyltransferase; (1 of 2) PTHR31953//PTHR31953:SF1 - FAMILY NOT NAMED // BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV2-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGCAGCAGCAGCGGCTACTGCTGCTGCTGCAGCTGTGGCGGCTGTAGC |
| Internal bar code: | TTGAGGACCTAGGTCTTTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1076 |
| LEAP-Seq percent confirming: | 13.0435 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGACAGCGGTGGTTCTG |
| Suggested primer 2: | AGGTGAGTATGATTGCGCGT |