| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.025588 |
| Chromosome: | chromosome 13 |
| Location: | 953617 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568100 | (1 of 4) 3.4.21.19 - Glutamyl endopeptidase / V8 proteinase | outside_mRNA | |
| Cre13.g568150 | CMAL1,SMM45,CMAL | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) 2.1.1.199 - 16S rRNA (cytosine(1402)-N(4))-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGCCTGGGGGTTCACGTGGCCCGTGCGTTTTAGATGCGCTTGGCAC |
| Internal bar code: | TCCGATCCGTGTAGAAAACAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 373 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTCGTCCAGAAAAGCGAA |
| Suggested primer 2: | TCTGGAGTGGCTGTGTTGAC |