| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025593 |
| Chromosome: | chromosome 16 |
| Location: | 177551 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g695100 | ACX4 | (1 of 1) PTHR10909//PTHR10909:SF331 - ELECTRON TRANSPORT OXIDOREDUCTASE // ACYL-COENZYME A OXIDASE 4, PEROXISOMAL; Putative Acyl-CoA oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGCGCGTGCCGGTACTTGTTGGATCCCCATCGGACACCGAAGAGTGCG |
| Internal bar code: | TCCAAGGTAAGCTGGTAAACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2173 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAGTTTACGGGTCGACC |
| Suggested primer 2: | TCACGACACACACACACACA |