Insertion junction: CLIP2.025601_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.025601
Chromosome:chromosome 12
Location:4577622
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g521650 (1 of 3) PTHR22753:SF1 - TRANSMEMBRANE PROTEIN 68 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCACGCCTACAGTGAGTACCCATCATCTCAGTCTCCTCTCCACGCATTA
Internal bar code:TTTTTTCAAAGGTGTGAAGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:70
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGCCAAACTGGTGTCATG
Suggested primer 2:ATCCACCTCCTCCTCCCTTC