Insertion junction: CLIP2.025622_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.025622
Chromosome:chromosome 16
Location:6109558
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g677350 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAAGACGTGGACACGCTGAATGAACGCAACCAGGTGGGGCCGTGGGCG
Internal bar code:GTAAAGCTGGTGCTTTGATCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:374
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAAGTACTCGTAGCGCTC
Suggested primer 2:ATTTGTGTACTCGTGCCGGT