| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.025633 |
| Chromosome: | chromosome 2 |
| Location: | 5629565 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAATGCCATGATTACGTTTTTGCTTTAGGGCGCCACAAGCACATGAAAG |
| Internal bar code: | AATTAGTTTGTATGTGGCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2266 |
| LEAP-Seq percent confirming: | 43.3333 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATGCATGATGTCAGGTG |
| Suggested primer 2: | TGACCACTGATCCTAGGGGG |