| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025643 |
| Chromosome: | chromosome 14 |
| Location: | 2457811 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623850 | (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGTTTCGTTTGAAACGATCGTTTATAAAGCTGAAATCCCGTTGAATGC |
| Internal bar code: | ATCTCGCGGTGTCAGATCCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1529 |
| LEAP-Seq percent confirming: | 35.7143 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGAGTGACCACAACGTAA |
| Suggested primer 2: | TTCCAAATCCGAGACAGGGC |