| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.025652 |
| Chromosome: | chromosome 6 |
| Location: | 1353380 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGCCTGAGAGGAACGCGTGTGCCCCTGCCCTGCACCATCTGAGAACG |
| Internal bar code: | GATCGAAGGCGCTCGCGGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 18.4211 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGTTCCAGACCCAACGC |
| Suggested primer 2: | CATCATACTGCGTGCGTGTG |