| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025655 |
| Chromosome: | chromosome 9 |
| Location: | 2525262 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392850 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGCGGTGCCGGCGGCAGTGTTGCGGGTGGCAAATCGCCGTGCTCCA |
| Internal bar code: | GCGGCGTATCGGATGCTAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4950 |
| LEAP-Seq percent confirming: | 74.5098 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTGCTCGGACGCATCAT |
| Suggested primer 2: | GGATGCTGAAGGTCAGGCTT |