Insertion junction: CLIP2.025662_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.025662
Chromosome:chromosome 3
Location:4942976
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g179150 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCTTTCGTGTTGACGTCTTTCGCCGGCGGCCCTGTGCGCTGGCTTGC
Internal bar code:TATCGACTATTGCAAGCTTGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1489
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAAACTGTCAAACCCCGC
Suggested primer 2:GCCATCCACAGGTCTGACTT