Insertion junction: CLIP2.025692_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.025692
Chromosome:chromosome 4
Location:1503471
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g213750 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACGTAGGGACCTTCGATATTTGAGCAAAACATGTTGAGTAATCGGGCG
Internal bar code:TACTGGGTTTTTATTCATTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:577
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AAACGGGTCTGGGAGCAAAA
Suggested primer 2:GGCAGTAGCACAACCTCCTT