Insertion junction: CLIP2.025706_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.025706
Chromosome:chromosome 6
Location:3800081
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278173 OPR59 OctotricoPeptide Repeat protein 59; (1 of 1) K08515 - vesicle-associated membrane protein 7 (VAMP7) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGACAGCGTCTGTAGCATCCGCGGCCAATGGTGGCGAGGAGGCTGAGGG
Internal bar code:TTAAAATACTCGTCACATAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1931
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CACCACCATCACCACTACCC
Suggested primer 2:GAGGTCGGTGCTTTTGAGGA