Insertion junction: CLIP2.025761_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.025761
Chromosome:chromosome 3
Location:8152028
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g200700 FAP166 (1 of 79) IPR011992 - EF-hand domain pair; Flagellar Associated Protein 166 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTAAGAACCTATGCGCACGTGGAACGAGTCAACAATCCCCACCCAGCA
Internal bar code:GCGCCCACATATACCATCACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1697
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCCTACTCGACATCTCC
Suggested primer 2:TCCGTGAAGGCTTTCAGTCC