| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025774 |
| Chromosome: | chromosome 3 |
| Location: | 6767256 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196651 | RAD10 | DNA repair protein; (1 of 1) K10849 - DNA excision repair protein ERCC-1 (ERCC1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTCCAGCTGCTGTCCCTGCTCCTGCCCCCGCCACGCCCGCGGCGCC |
| Internal bar code: | AGAACAGTAACTTTGTCTTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3602 |
| LEAP-Seq percent confirming: | 89.4118 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTACTCTTGGCTGCACC |
| Suggested primer 2: | ATGGGCCTGCAAAACCGATA |