| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025776 |
| Chromosome: | chromosome 16 |
| Location: | 6491120 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674850 | (1 of 1) PTHR14625//PTHR14625:SF3 - MICROCEPHALIN // MICROCEPHALIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGCGCAACACCGGAAACCGAGTGTGGCTGTGCGCCAGCACGCAGCAC |
| Internal bar code: | GGGGCTCAGAGACTTCGGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1174 |
| LEAP-Seq percent confirming: | 45.4545 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTTGACGGGCCATTTGC |
| Suggested primer 2: | TTCCTCTGCTGAAGCCACTG |