| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025805 |
| Chromosome: | chromosome 12 |
| Location: | 735509 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492700 | FAS1 | (1 of 10) PTHR10900 - PERIOSTIN-RELATED; Fasciclin-like protein 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTAGGACCCCTCCGCCTTGACGGCCGCCAGCAGTGTGTTGACGTCGGT |
| Internal bar code: | TGATATGGTAGTCGACCAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4392 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGCCTCAACCTTCAGAT |
| Suggested primer 2: | GCAACGCAAAGATCAAGCCA |