Insertion junction: CLIP2.025809_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.025809
Chromosome:chromosome 9
Location:2322831
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394350 NUP155 Nucleoporin 155; (1 of 1) K14312 - nuclear pore complex protein Nup155 (NUP155, NUP170, NUP157) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCCCAGGCGCACCGCATGGGCGCGCGGTCCGATCCTGCGCCAATCCT
Internal bar code:ACCGGTAGCCACAGGGGTAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:581
LEAP-Seq percent confirming:93.3333
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCCAAGATCGTCTACCGT
Suggested primer 2:GGGCTGTGTACGTGTGTGTA