Insertion junction: CLIP2.025821_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.025821
Chromosome:chromosome 2
Location:3122872
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g800210 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACAAACCCAATCCTTGCACCGGTGCTGCGTCCGCCGCACCCACGAAG
Internal bar code:CAGTGAGGGAGGCCAAGTAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2985
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:CCATCGAGCGCAAAAACACA
Suggested primer 2:GAGAAGTGCGCGAGGTATGA