Insertion junction: CLIP2.025833_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.025833
Chromosome:chromosome 2
Location:3267093
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g097250 transportin-3 family protein; (1 of 1) K15436 - transportin-3 (TRPO3, MTR10) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTATACAGAACAGCGCCGCCTCCGCCGTCCGCCAGTCGTAGCTGCTGC
Internal bar code:GGGTTCCCGGTAGCCAGGTATC

Confirmation - LEAP-Seq

LEAP-Seq distance:318
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGCACACAAGCACACTC
Suggested primer 2:AAGATTCTGCCAACGCTCCA