Insertion junction: CLIP2.025838_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.025838
Chromosome:chromosome 13
Location:4307510
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g592550 (1 of 2) PF13661 - 2OG-Fe(II) oxygenase superfamily (2OG-FeII_Oxy_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCCAGCTTGGCTGAGGAGGGCGCCCATGATGTCTCGTGCGTGGCGAGC
Internal bar code:TACGAGGCGGTTTGCACTCCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1547
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GGTATGTTGGCGGGAGAACA
Suggested primer 2:GCGCAAATCCCGGTATCCTA