| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025850 |
| Chromosome: | chromosome 12 |
| Location: | 521075 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801346 | (1 of 10) PF00176//PF00271 - SNF2 family N-terminal domain (SNF2_N) // Helicase conserved C-terminal domain (Helicase_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTAGTTTGGGCTGGTATCTACAACCACCCCCGCCCCTCCCGCTGCCG |
| Internal bar code: | AGGGACTAGCCACTCTTTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1165 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCTACCCAGCTACTAGC |
| Suggested primer 2: | TAGCGGAGTAGACAGCAGGT |