Insertion junction: CLIP2.025955_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.025955
Chromosome:chromosome 13
Location:2546712
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g580250 (1 of 1) PF03941 - Inner centromere protein, ARK binding region (INCENP_ARK-bind) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTCCCTTCCGCCTCGGCCCTCTCGCCCCAGGTCGGACAGCGACGAAGA
Internal bar code:TCTAAGGATAGCTTTAAATCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:4804
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:74
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAACACAGCACTCCACCA
Suggested primer 2:GAGCAGGGCTTGTTTGCAAA