Insertion junction: CLIP2.025958_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.025958
Chromosome:chromosome 15
Location:1163235
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g639150 MDM38 (1 of 1) K17800 - LETM1 and EF-hand domain-containing protein 1, mitochondrial (LETM1, MDM38); Mitochondrial distribution and morphology protein 38 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTTCTACAACCTTGCTTCCTGATACTTCATCCTGTGATGGCTGTAGT
Internal bar code:TTACATGAATCTTTGCCGACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1001
LEAP-Seq percent confirming:65.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TACCACACACCGAAACCGAG
Suggested primer 2:GCGCGAGGAAGAGATCATCA