| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.025979 |
| Chromosome: | chromosome 15 |
| Location: | 1270128 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638304 | NCL30,OPR74 | Nuclear Control of chloroplast Like 30 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCGGACGGAGGTGGTGGTGGAGGGCGTGCTGGGGCGTGTGGATATTG |
| Internal bar code: | TGTCCGAACGGTATAGTCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1796 |
| LEAP-Seq percent confirming: | 18.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCATGGGGGCTTGCTAA |
| Suggested primer 2: | GACCTTTGCAACAGCCTGTG |