Insertion junction: CLIP2.025984_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.025984
Chromosome:chromosome 13
Location:2903045
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g583400 OPR57 OctotricoPeptide Repeat protein 57; (1 of 781) IPR000104 - Antifreeze protein, type I CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCAGCGCTGTCAGGACTGCGCCCCCCTTGGTTGGTGAGGCTACATTC
Internal bar code:AAATGCAGGTATTTGGGCTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2456
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAGTGCTCGGATGTTTGG
Suggested primer 2:GACGTGGATGTGGTTCATGC