| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025988 |
| Chromosome: | chromosome 7 |
| Location: | 767466 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317864 | (1 of 15) PF13365 - Trypsin-like peptidase domain (Trypsin_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTAGGCGACAATCCTCTTGTTACATTGGTGCGAAATGCCAGGAGTCA |
| Internal bar code: | AATCGCGGTAATGGGCGGAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2099 |
| LEAP-Seq percent confirming: | 76.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTAGCAAGCAATCCACA |
| Suggested primer 2: | TGCGTGCTGGTTCAATGTTG |