Insertion junction: CLIP2.025999_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.025999
Chromosome:chromosome 10
Location:2981496
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g440350 (1 of 3) PF07887 - Calmodulin binding protein-like (Calmodulin_bind) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGGAGCTGCTCAAGACCACCACGTCTGGCATGTTGAAGCCAACCTCCA
Internal bar code:GGAGATAGCGGGATGCGTAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:4654
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:AACTCCCGATACGTGCGATC
Suggested primer 2:CCCTGCCCTTTACAAACAGC