Insertion junction: CLIP2.026001_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.026001
Chromosome:chromosome 16
Location:1123507
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g649800 PPR3,CYCR6 (1 of 1) PF02984//PF13041 - Cyclin, C-terminal domain (Cyclin_C) // PPR repeat family (PPR_2); PentatricoPeptide Repeat protein 3 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCCTGGAGACGCTGCGCGAGATGGTGAGTCCTTTCCACGCGTGTTGAC
Internal bar code:TTGACTAGTGCTCGGTGTGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:559
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCATAAGGACCCCGATGTCG
Suggested primer 2:TCCTTGTGTCGACTCGTGTG