Insertion junction: CLIP2.026004_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.026004
Chromosome:chromosome 9
Location:1395566
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g400150 (1 of 2) PF13415//PF13418 - Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCACAACTCCAGTTGCGCAGCCCCTTACCACCGGCGCCCGCTTTGCCT
Internal bar code:CATCGTTGCACCTGGTATGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1206
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTCCAAGCCTCAATGGTG
Suggested primer 2:CCCTACGCATGCTCGAAGAT