Insertion junction: CLIP2.026015_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.026015
Chromosome:chromosome 3
Location:3889945
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g170625 (1 of 45) IPR023329 - Chlorophyll a/b binding protein domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCGCCGTAGCTGCCACCACCCCCACCACCACCACCACCAGCCATAGC
Internal bar code:TATCAAGGTCTTTGTTTGAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2149
LEAP-Seq percent confirming:96.875
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TGATACAGTGCGGCCTGTTT
Suggested primer 2:CTGAAGAGGCAAGTCAGGCA