| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.026026 |
| Chromosome: | chromosome 16 |
| Location: | 3973952 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689700 | HPR5 | (1 of 1) 1.1.1.272 - D-2-hydroxyacid dehydrogenase (NADP(+)); Hydroxypyruvate reductase 5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTGATGCAGCAATCAGGAAGTTCCAGGACTCACCCCAGCCGCGCCGTC |
| Internal bar code: | GACAAGTGTAGCAACGCCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTGCTTTATGGGACCTT |
| Suggested primer 2: | CGATTAGGAAGCCCCCTCAC |