Insertion junction: CLIP2.026029_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.026029
Chromosome:chromosome 13
Location:589977
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g565517 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAAACGCAGAACACTAACGCGTCCGAAACATCCAACCCCCAACCTGTC
Internal bar code:TAATGTCGTGTGATGGCAGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:4838
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:79
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:79

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTCCAGCACCGTAACGAG
Suggested primer 2:CGGACAGCATGCAATACACG