| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.026041 |
| Chromosome: | chromosome 9 |
| Location: | 2560755 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392652 | (1 of 1) 1.5.1.22 - Strombine dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGATCAGTAACAGCCGCCGCAGCAGCTGTTGCAGCAGCTACAGTCGCC |
| Internal bar code: | CGTGTGACTTCTTGTGGGCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 63.6364 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGCCGTGTTCAAGAGTG |
| Suggested primer 2: | TCTGTCTGCGCGTGTTTACT |