Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.026085 |
Chromosome: | chromosome 10 |
Location: | 5388086 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g457100 | (1 of 1) 1.13.11.79 - 5,6-dimethylbenzimidazole synthase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCTGACGCACTACTGCCGGTGCGCACAGGCGGCCGCTGCCGGCAGC |
Internal bar code: | AGCTCTGAAGTAAAATATAGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 4168 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 100 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 100 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAATTGTCTGCGCGGAGATG |
Suggested primer 2: | GTTACCCCCTTCTTCCCGTG |