Insertion junction: CLIP2.026129_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.026129
Chromosome:chromosome 14
Location:2717227
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g625901 (1 of 3) PF01985 - CRS1 / YhbY (CRM) domain (CRS1_YhbY) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACGCCGCACACCAGCGAAGGCAGTGTCGGCGGCCCGTGCTTGCCACAG
Internal bar code:TTGTTATTTAGCTGTGACACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1515
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAGTAACTGCGGTGTCCA
Suggested primer 2:TCGTGCGAGTCAACATGTCA