Insertion junction: CLIP2.026150_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.026150
Chromosome:chromosome 3
Location:2538073
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g159650 EFG6 (1 of 1) PTHR23115//PTHR23115:SF96 - TRANSLATION FACTOR // SUBFAMILY NOT NAMED; Putative organellar translation elongation factor EFG/EF2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAATGTACCGGGCCACCTGGTAACACCAGCCCCGCACCCAGGCCCAGCA
Internal bar code:TCGCTATGCGGTATAGGTATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2217
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGACGTGAATGCCCAACC
Suggested primer 2:GCGTATGTCGAGCTAACCGA