| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.026151 |
| Chromosome: | chromosome 3 |
| Location: | 2674665 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160850 | (1 of 2) PTHR35100:SF1 - F15H11.13 PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACCTTGCGGGCCGCCGGCCGGAAGTGCTTGCCCCAGGAGGCGTGGTA |
| Internal bar code: | GTGCTGAATTAATCCATTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2609 |
| LEAP-Seq percent confirming: | 94.5946 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGAGGGAGAGATTCGGA |
| Suggested primer 2: | GTCAAGTCCCACCAGGTACG |