Insertion junction: CLIP2.026164_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.026164
Chromosome:chromosome 8
Location:3487509
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g378850 RPPK1 (1 of 1) K00948 - ribose-phosphate pyrophosphokinase (PRPS, prsA); Ribose-phosphate pyrophosphokinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAAACTTGCGCACTTTTGGCCCTTGCAGCCGACACCCACCCATCCACG
Internal bar code:CAGTCTGCAAAGTTTCACTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1992
LEAP-Seq percent confirming:97.9167
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:AACTCTGGACGTGACGACAC
Suggested primer 2:GTATAAGACCTCGGGCTGCC