Insertion junction: CLIP2.026166_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.026166
Chromosome:chromosome 1
Location:2336213
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGCTATAGCAGCTATTAGCAACAAGTCAGCGAGTGTTCTGCTTGCGTG
Internal bar code:TTCGGGTAGTTCTCGTAATTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2918
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AACGTCAGTAGCACGCAAGA
Suggested primer 2:CAGGTGTGAAGTACGGCAGT