| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.026172 |
| Chromosome: | chromosome 17 |
| Location: | 720886 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700750 | GFY3 | (1 of 5) PTHR30178 - INNER MEMBRANE PROTEIN YAAH; Putative acetate transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTACAGAAACACAAAGGCGGCGTAGATGGCGCTGGAGCCGCAGAAGAA |
| Internal bar code: | TATATTGTGGATCAAGATGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2697 |
| LEAP-Seq percent confirming: | 91.2281 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACACCGTTGCAACACTG |
| Suggested primer 2: | CGTATGTAAACGCAGGCACG |